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384 solid pin multi blot replicator tool  (V&P Scientific)


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    Structured Review

    V&P Scientific 384 solid pin multi blot replicator tool
    384 Solid Pin Multi Blot Replicator Tool, supplied by V&P Scientific, used in various techniques. Bioz Stars score: 94/100, based on 136 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/384+solid+pin+multi+blot+replicator/VP+384/us11229649-236-1-7
    Average 94 stars, based on 136 article reviews
    384 solid pin multi blot replicator tool - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Drug Repurposing Screen Reveals FDA-Approved Inhibitors of Human HMG-CoA Reductase and Isoprenoid Synthesis That Block Cryptosporidium parvum Growth
    Article Snippet: The compounds were further diluted by a factor of 1:1.4 in 100% DMSO, arrayed into the center 308 wells of V-bottom polypropylene 384-well source plates (Whatman), and stored at 80°C until use. .. A 384 solid pin Multi-Blot replicator (V&P Scientific) was used to transfer approximately 120 nl of the compound from the source plate to the assay plate for a final concentration of approximately 10 M. Controls on each compound source plate included wells containing DMSO (vehicle) only and wells containing nitazoxanide at a concentration that resulted in delivery of the 90% inhibitory concentration (IC90) of nitazoxanide to ensure consistent loading across the pin transfer tool. (ii) Secondary assays. ..

    Article Title: Drug Repurposing Screen Reveals FDA-Approved Inhibitors of Human HMG-CoA Reductase and Isoprenoid Synthesis That Block Cryptosporidium parvum Growth
    Article Snippet: The compounds were further diluted by a factor of 1:1.4 in 100% DMSO, arrayed into the center 308 wells of V-bottom polypropylene 384-well source plates (Whatman), and stored at −80°C until use. .. A 384 solid pin Multi-Blot replicator (V&P Scientific) was used to transfer approximately 120 nl of the compound from the source plate to the assay plate for a final concentration of approximately 10 μM. ..

    Saline:

    Article Title: Efficient Generation of Cardiac Purkinje-like Cells from Embryonic Stem Cells by Activating cAMP Signaling.
    Article Snippet: Strategies to derive cardiac conduction system (CCS) cells including Purkinje cells (PC) would facilitate models for mechanistic studies and drug discovery, and also provide new cellular materials for regenerative therapies.. However, using current cardiac differentiation protocols, the differentiation efficiency of CCS cells is extremely low, typically below 1% of the culture.. High-throughput chemical screening is a powerful strategy for identifying small molecules that can activate signaling pathways to enhance embryonic stem cell (ESC) differentiation.

    Recombinant:

    Article Title: Efficient Generation of Cardiac Purkinje-like Cells from Embryonic Stem Cells by Activating cAMP Signaling.
    Article Snippet: Strategies to derive cardiac conduction system (CCS) cells including Purkinje cells (PC) would facilitate models for mechanistic studies and drug discovery, and also provide new cellular materials for regenerative therapies.. However, using current cardiac differentiation protocols, the differentiation efficiency of CCS cells is extremely low, typically below 1% of the culture.. High-throughput chemical screening is a powerful strategy for identifying small molecules that can activate signaling pathways to enhance embryonic stem cell (ESC) differentiation.

    Sterility:

    Article Title: Efficient Generation of Cardiac Purkinje-like Cells from Embryonic Stem Cells by Activating cAMP Signaling.
    Article Snippet: Strategies to derive cardiac conduction system (CCS) cells including Purkinje cells (PC) would facilitate models for mechanistic studies and drug discovery, and also provide new cellular materials for regenerative therapies.. However, using current cardiac differentiation protocols, the differentiation efficiency of CCS cells is extremely low, typically below 1% of the culture.. High-throughput chemical screening is a powerful strategy for identifying small molecules that can activate signaling pathways to enhance embryonic stem cell (ESC) differentiation.

    FACS:

    Article Title: Efficient Generation of Cardiac Purkinje-like Cells from Embryonic Stem Cells by Activating cAMP Signaling.
    Article Snippet: Strategies to derive cardiac conduction system (CCS) cells including Purkinje cells (PC) would facilitate models for mechanistic studies and drug discovery, and also provide new cellular materials for regenerative therapies.. However, using current cardiac differentiation protocols, the differentiation efficiency of CCS cells is extremely low, typically below 1% of the culture.. High-throughput chemical screening is a powerful strategy for identifying small molecules that can activate signaling pathways to enhance embryonic stem cell (ESC) differentiation.

    Blocking Assay:

    Article Title: Efficient Generation of Cardiac Purkinje-like Cells from Embryonic Stem Cells by Activating cAMP Signaling.
    Article Snippet: Strategies to derive cardiac conduction system (CCS) cells including Purkinje cells (PC) would facilitate models for mechanistic studies and drug discovery, and also provide new cellular materials for regenerative therapies.. However, using current cardiac differentiation protocols, the differentiation efficiency of CCS cells is extremely low, typically below 1% of the culture.. High-throughput chemical screening is a powerful strategy for identifying small molecules that can activate signaling pathways to enhance embryonic stem cell (ESC) differentiation.

    other:

    Article Title: Stress sensor Ire1 deploys a divergent transcriptional program in response to lipid bilayer stress
    Article Snippet: The genetic screen was condensed with the 384 Solid Pin Multi-Blot Replicator (V&P Scientific) and performed in 384-format until analysis.



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    V&P Scientific 384 solid pin multi blot replicator
    Construction of the <t>384-format</t> mutant array guided by the library copier VP381 Yeast deletion library colonies grown on the source plates (YPD agar plate containing G418) were created by inoculation from frozen glycerol stock in 96-format arrays. Before the subsequent pinning steps, fit the destination plate (YPD agar plate containing G418) into the middle of the library copier VP381. To condense four 96-format arrays into a single 384-format array, pin the colonies on the first source plate using a sterile 96-pin <t>replicator</t> and replicate onto the destination plate aligned to the ‘A’ alignment holes. Repeat the pinning step with the next three source plates aligned to the ‘B’, ‘C’, and ‘D’ alignment holes and transfer to the same destination plate. The resulting 384-format mutant array will then be used for subsequent SGA steps to generate the final mutant array expressing the UPR sensors and reporters. Each 384-format array can be expanded to four 96-format liquid culture for flow cytometry acquisition.
    384 Solid Pin Multi Blot Replicator, supplied by V&P Scientific, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/384+solid+pin+multi+blot+replicator/VP+384/pmc08496318-81-0-6
    Average 94 stars, based on 1 article reviews
    384 solid pin multi blot replicator - by Bioz Stars, 2026-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    Construction of the 384-format mutant array guided by the library copier VP381 Yeast deletion library colonies grown on the source plates (YPD agar plate containing G418) were created by inoculation from frozen glycerol stock in 96-format arrays. Before the subsequent pinning steps, fit the destination plate (YPD agar plate containing G418) into the middle of the library copier VP381. To condense four 96-format arrays into a single 384-format array, pin the colonies on the first source plate using a sterile 96-pin replicator and replicate onto the destination plate aligned to the ‘A’ alignment holes. Repeat the pinning step with the next three source plates aligned to the ‘B’, ‘C’, and ‘D’ alignment holes and transfer to the same destination plate. The resulting 384-format mutant array will then be used for subsequent SGA steps to generate the final mutant array expressing the UPR sensors and reporters. Each 384-format array can be expanded to four 96-format liquid culture for flow cytometry acquisition.

    Journal: STAR Protocols

    Article Title: A high-throughput genetic screening protocol to measure lipid bilayer stress-induced unfolded protein response in Saccharomyces cerevisiae

    doi: 10.1016/j.xpro.2021.100868

    Figure Lengend Snippet: Construction of the 384-format mutant array guided by the library copier VP381 Yeast deletion library colonies grown on the source plates (YPD agar plate containing G418) were created by inoculation from frozen glycerol stock in 96-format arrays. Before the subsequent pinning steps, fit the destination plate (YPD agar plate containing G418) into the middle of the library copier VP381. To condense four 96-format arrays into a single 384-format array, pin the colonies on the first source plate using a sterile 96-pin replicator and replicate onto the destination plate aligned to the ‘A’ alignment holes. Repeat the pinning step with the next three source plates aligned to the ‘B’, ‘C’, and ‘D’ alignment holes and transfer to the same destination plate. The resulting 384-format mutant array will then be used for subsequent SGA steps to generate the final mutant array expressing the UPR sensors and reporters. Each 384-format array can be expanded to four 96-format liquid culture for flow cytometry acquisition.

    Article Snippet: 384 Solid Pin Multi-Blot Replicator , V&P Scientific , VP 384F.

    Techniques: Mutagenesis, Sterility, Expressing, Flow Cytometry

    Journal: STAR Protocols

    Article Title: A high-throughput genetic screening protocol to measure lipid bilayer stress-induced unfolded protein response in Saccharomyces cerevisiae

    doi: 10.1016/j.xpro.2021.100868

    Figure Lengend Snippet:

    Article Snippet: 384 Solid Pin Multi-Blot Replicator , V&P Scientific , VP 384F.

    Techniques: Recombinant, Clone Assay, Software, Sterility, Microscopy